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KMID : 0391319920020020125
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Korean Journal of Biological Response Modifiers
1992 Volume.2 No. 2 p.125 ~ p.134
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Inhibition of Tumor Cell Proliferation of Lymphokine-Activated Killer (LAK) Cell Culture Supernatant
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김영일
남상윤/김시영/윤휘중/조경삼/김현숙
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Abstract
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It is well known-that lymphokine-activated killer (LAK)cell can destory various types of tumor cells and the fact has been applied to the adoptive immunotherapy of cancers. The antitumor activity of LAK cells has been clarified but the effect of
the
supernatant of LAK cell has been clarified but the effect of the supernatant of LAK cell culture has no clear conclusion yet.
Human fibroblasts from normal cell origin and human tumor cell lines (HeLa cell, MCF-7 cell) were employed to measure and confirm the cytotoxicity of LAK cells and its culture supernatant.
The cytotoxicity was measured through induction culture and expansion culture to find out the relationship between the two activities by comparing the activity of LAK cells and its culture superantant.
The cytotoxicity of LAK cell culture supernatant has been increased continuously upto 4 days after exposing LAK cell culture supernatant to tumor cells and since then it appeared as a plateau but there was no effect upon the normal cells.
In terms of cytotoxicity the effect of LAK cells were the highest after 10 day-and 13 day-expansion culture compared to 4 day-induction culture. However, cytotoxicity of culture supernatnant showed the highest in the 4 day-induced supernatant but
the
cytotoxicity of the culture supernatant was declined Apparently the cytotoxicity between LAK cells and culture supernatant was very different.
From the results of the above, cytotoxicity of LAK cell culture supernatant was not considered to be originated from induced LAK cells, Possibly it may be originating from the process of LAK cell differentiation.
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